Journal: Frontiers in Cell and Developmental Biology
Article Title: Degradable fibrin hydrogels for transplantation of iPSC-derived retinal pigment epithelial cell monolayers
doi: 10.3389/fcell.2025.1739620
Figure Lengend Snippet: Histology following RPE debridement and RPE-fibrin transplantation. H&E-stained sections from a control region (A) , debridement zone containing RPE transplant (B) , and debridement zone (C) of Pig 2 sacrificed 2 months after receiving the RPE + fibrin transplant. Transplanted RPE in B exhibit mild depigmentation but are in contact with photoreceptor outer segments (OS) which are shortened relative to control regions. There is also thickening of choroid (Ch), loss of choroidal pigmentation, and cellular infiltration. The debridement zone (C) exhibits significant disruption of retinal layering with tubulation of surviving photoreceptors, choroidal thickening, and absence of RPE. The presence of transplanted RPE was verified by staining for the human specific RPE antibody Tra-1-85 (Green in D–G ) which recognizes CD147. Note Tra-1-85 does not stain RPE in the control region of the pig eye (D) but does stain transplanted cells (E) , RPE in a human eye (F) , and the RPE component of RPE-fibrin in vitro (G) . GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; OS, outer segment; Ch, choroid. Nuclei are stained in (D–G) with DAPI (Blue).
Article Snippet: Immunofluorescence was performed, as described previously ( ; ), using rabbit polyclonal antibody Pab125 to detect Best1 , CD147/EMMPRIN using mouse monoclonal antibody Tra-1-85 (R&D Systems, cat# MAB3195, RRID: AB_2066681), or CRALBP using mouse monoclonal antibody B2 (Novus, cat# NB100-74392, RRID: AB_1048601).
Techniques: Transplantation Assay, Staining, Control, Disruption, In Vitro